![[Gln8]-C517 (LH-RH), chicken](/upload/1124/V30469.png)
| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
GnRH Receptor (gonadotropin-releasing hormone receptor). As an avian hypothalamic peptide, it targets the GnRH receptor to stimulate the release of gonadotropins from the anterior pituitary.
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|---|---|
| ln Vitro |
[Gln8]-C517 (LH-RH), chicken stimulates the release of gonadotropins from the anterior pituitary, thus regulating reproductive functions. As an avian hypothalamic peptide, its primary activity is the stimulation of gonadotropin release.
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| ln Vivo |
No specific in vivo activity data is available beyond its described function as an avian hypothalamic peptide that stimulates gonadotropin release. It is used in research to study reproductive function and the regulation of gonadotropin secretion.
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| Enzyme Assay |
In vitro receptor binding assays for [Gln8]-C517 (LH-RH), chicken involve measuring its affinity for the GnRH receptor using radioligand displacement or cell-based functional assays. Receptor activation can be assessed by measuring downstream signaling (e.g., calcium mobilization, cAMP levels) in cells expressing the GnRH receptor.
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| Cell Assay |
The in vitro cellular activity of [Gln8]-C517 (LH-RH), chicken is evaluated in pituitary cell cultures or cell lines expressing the GnRH receptor. Cells are treated with the peptide, and gonadotropin release (LH and FSH) is measured using immunoassays. Receptor activation and downstream signaling pathways are also assessed.
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| Animal Protocol |
In vivo animal studies with [Gln8]-C517 (LH-RH), chicken would typically involve administration to avian species or rodent models to study reproductive function. The peptide would be administered via injection, and endpoints would include measurement of gonadotropin levels, reproductive organ weights, and fertility parameters.
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| ADME/Pharmacokinetics |
[Gln8]-C517 (LH-RH), chicken has a molecular formula of C54H71N15O14 and a molecular weight of 1154.23. The sequence is (pGLU)-His-Trp-Ser-Tyr-Gly-Leu-Gln-Pro-Gly. Solubility: H2O: 100 mg/mL (86.64 mM). Storage: powder at -80degC for 2 years; in solvent at -80degC for 6 months.
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| Toxicity/Toxicokinetics |
No specific toxicological data is available for [Gln8]-C517 (LH-RH), chicken. As a research peptide intended for laboratory use, standard safety precautions should be observed. No clinical toxicity studies have been reported.
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| Additional Infomation |
[Gln8]-C517 (LH-RH), chicken is an avian hypothalamic peptide that stimulates the release of gonadotropins from the anterior pituitary, thereby regulating reproductive functions. It is a research tool for studying reproductive endocrinology and the regulation of gonadotropin secretion. It is not approved for clinical use.
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| Molecular Formula |
C54H71N15O14
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|---|---|
| Molecular Weight |
1154.23000
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| Exact Mass |
1153.53
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| CAS # |
47922-48-5
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| PubChem CID |
123777
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.398g/cm3
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| Boiling Point |
1812ºC at 760 mmHg
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| Flash Point |
1049.7ºC
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| Index of Refraction |
1.629
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| LogP |
0.619
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| Hydrogen Bond Donor Count |
15
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
30
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| Heavy Atom Count |
83
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| Complexity |
2340
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| Defined Atom Stereocenter Count |
8
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| SMILES |
CC(C[C@H](NC(CNC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H]1CCC(N1)=O)=O)CC2=CN=CN2)=O)CC3=CNC4=CC=CC=C34)=O)CO)=O)CC5=CC=C(O)C=C5)=O)=O)C(N[C@H](C(N6CCC[C@H]6C(NCC(N)=O)=O)=O)CCC(N)=O)=O)C
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| InChi Key |
WPKQYFUSPONRAT-BXXNOCLASA-N
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| InChi Code |
InChI=1S/C54H71N15O14/c1-28(2)18-37(49(78)64-36(13-15-43(55)72)54(83)69-17-5-8-42(69)53(82)59-24-44(56)73)63-46(75)25-60-47(76)38(19-29-9-11-32(71)12-10-29)65-52(81)41(26-70)68-50(79)39(20-30-22-58-34-7-4-3-6-33(30)34)66-51(80)40(21-31-23-57-27-61-31)67-48(77)35-14-16-45(74)62-35/h3-4,6-7,9-12,22-23,27-28,35-42,58,70-71H,5,8,13-21,24-26H2,1-2H3,(H2,55,72)(H2,56,73)(H,57,61)(H,59,82)(H,60,76)(H,62,74)(H,63,75)(H,64,78)(H,65,81)(H,66,80)(H,67,77)(H,68,79)/t35-,36-,37-,38-,39-,40-,41-,42-/m0/s1
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| Chemical Name |
(2S)-N-[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[2-[[(2S)-1-[[(2S)-5-amino-1-[(2S)-2-[(2-amino-2-oxoethyl)carbamoyl]pyrrolidin-1-yl]-1,5-dioxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxopropan-2-yl]amino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]-5-oxopyrrolidine-2-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~86.64 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8664 mL | 4.3319 mL | 8.6638 mL | |
| 5 mM | 0.1733 mL | 0.8664 mL | 1.7328 mL | |
| 10 mM | 0.0866 mL | 0.4332 mL | 0.8664 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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